Internal Transcribed Spacer (ITS) as Dna Barcoding to Identify Fungal Species: a Review
نویسندگان
چکیده
منابع مشابه
Limits of nuclear ribosomal DNA internal transcribed spacer (ITS) sequences as species barcodes for Fungi.
In PNAS, the work by Schoch et al. (1) proposed nuclear ribosomal DNA internal transcribed spacer (ITS) sequences as the sole universal barcode for fungi. The work by Schoch et al. (1) stated that “the proposal will satisfy most fungal biologists but not all” (1). I am among those biologists who are not so satisfied and not really surprised either, because ITS has been the most widely used mark...
متن کاملInternal Transcribed Spacer (its), an Ideal Dna Barcode for Species Discrimination in Crawfurdia Wall. (gentianaceae)
BACKGROUND DNA barcoding is a technique used to identify species based on species-specific differences in short regions of their DNA. It is widely used in species discrimination of medicinal plants and traditional medicines. MATERIALS AND METHODS In the present study, four potential DNA barcodes, namely rbcL, matK, trnH-psbA and ITS (nuclear ribosomal internal transcribed spacer) were adopted...
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Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region of the mitochondrial cytochrome c oxidase subunit 1 used as the animal barcode was excluded as a potential marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variabl...
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Advances in molecular technology show great potential for the rapid detection and identification of fungi for medical, scientific and commercial purposes. Numerous targets within the fungal genome have been evaluated, with much of the current work using sequence areas within the ribosomal DNA (rDNA) gene complex. This section of the genome includes the 18S, 5.8S and 28S genes which code for rib...
متن کاملDNA barcoding of oomycetes with cytochrome c oxidase subunit I and internal transcribed spacer
Oomycete species occupy many different environments and many ecological niches. The genera Phytophthora and Pythium for example, contain many plant pathogens which cause enormous damage to a wide range of plant species. Proper identification to the species level is a critical first step in any investigation of oomycetes, whether it is research driven or compelled by the need for rapid and accur...
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ژورنال
عنوان ژورنال: Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
سال: 2016
ISSN: 2406-9272,2089-5690
DOI: 10.15578/squalen.v11i2.213